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KMID : 1025520060480060805
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Journal of Animal Science and Technology
2006 Volume.48 No. 6 p.805 ~ p.812
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Development of In vitro Fertilized Bovine Embryos in Medium Supplemented with Matrigel
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Kim Dong-Hoon
Kim Se-Woong
Lee Min-Jung
Hwang In-Sun
Bae Sung-Hoon
Yang Byong-Chul
Im Gi-Sun
Seong Hwan-Hoo
Yang Boh-Suck
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Abstract
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The purpose of this study was to investigate the effect of Matrigel on the development of bovine embryos after in vitro fertilization. Bovine embryos were cultured in Ⅰ) SOF+ 0.8% BSA(SOF-B), Ⅱ) SOF+ 0.8% BSA plus 0.8% Matrigel(SOF-M) and III) SOF+0.8% BSA and 10% FBS(SOF-BF). The addition of Matrigel appeared not to increase the proportion of blastocysts (SOF-B, 26.6%; SOF-M, 28.2%; SOF-BF, 26.2%). However, the proportion of hatched blastocysts were significantly increased(P<0.05) by Matrigel(SOF-B, 23.7%; SOF-M, 48.7%; SOF-BF, 18.5%). The means of cell number blastocyst was not significantly different among the treatment groups(SOF-B, 172.7±35.5; SOF-M, 175.1±37.4; SOF-BF, 172.8±38.1). The proportion of apoptotic cells in blastocyst was also found to be not significant among the treatment groups(SOF-B, 3.6±3.2%; SOF-M, 4.3±2.6%; SOF-BF, 4.9±4.3%). In this experiment, Matrigel appeared to support embryonic hatching of bovine embryos. Results suggest that Matrigel, as extracellular matrix components, may be another avenue for formulating more physiological culture system in serum-free culture.
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KEYWORD
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Matrigel, Bovine, In vitro fertilization, Hatched blastocyst
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